SYNERGISM TEST OF DATE (PHOENIX DACTYLIFERA L.) AND RAW TEMPE ON ANTIOXIDANT ACTIVITY
DOI:
https://doi.org/10.47522/jmk.v1iIAHSC.122Keywords:
Dates, Tempeh, Antioxidant activity, Synergistic effect, DPPHAbstract
Introduction: Dates and tempeh are foods that both contain antioxidant compounds. Antioxidant compounds such as flavonoids and isoflavones are known to be found in dates and tempeh. This study was conducted to see how much antioxidant activity contained in dates and tempeh and how the effect of dates and tempeh consumed simultaneously on the increase in total antioxidant activity.
Method: This study uses a direct experimental design with prospective data collection. The selected samples were dates with the type of sukkari and tempeh wrapped in plastic. Data processing was carried out using a simple linear regression statistical method.
Results: The DPPH test method with UV-Visible Spectrophotometry instrument showed the results of the % inhibition value of dates fruit of 39.99% and tempeh of 24.52%. Testing the synergistic effect using 7 treatments showed that the treatment with a ratio of 50:50 had a higher % inhibition value than the other treatments, which showed that consuming dates and tempeh in a ratio of 50:50 could provide a synergistic effect on antioxidant activity.
Conclusion The results obtained indicate that both samples, both dates and tempeh have high antioxidant activity, consuming both simultaneously can increase antioxidant activity. So it can be concluded that there is an effect on the synergistic effect of antioxidant activity of dates and tempeh which are consumed simultaneously. It is recommended to conduct research on the antioxidant activity of dates and tempeh using other test methods, and further research on the formulation of the preparation or clinical trials using mice.
References
Abdillah, M., Nazilah, NRK, & Agustina, E. (2017). Identification Of Active Compounds In Methanol Extract Of Ajwa Date Fruit Meat (Phoenix dactylvera L.) Lecturer / Biology Study Program at UIN Sunan Ampel Abdillah et al, Identification of Active Compounds Abdillah et al., Identification of Active Compounds. April, 69–74.
Barus, T., Titarsole, NN, Mulyono, N., & Prasasty, VD (2019). Tempeh Antioxidant Activity using DPPH Method: Effects of Fermentation, Processing, and Microorganisms. Journal of Food Engineering and Technology, 8(2), 75–80. https://doi.org/10.32732/jfet.2019.8.2.75
Istiani, Y. (2010). Ethanol extract of tempeh made from koro sword (Canavalia ensiformis). Postgraduate Journal of Sebelas Maret University Surakarta, 90.
Ministry of Health of the Republic, I. (2017). Indonesian Herbal Pharmacopoeia (2nd ed.). Indonesian Ministry of Health.
Linga, L. (2012). The Healing Power of Anti-oxidants. Jakarta: Elex Media Komputindo.
Maulana, MSR (2017). Indonesian Traditional Medicine Herb Formulary. Decree of the Minister of Health of Indonesia. Ekp, 13(3), 1576–1580.
Mawaddah, N., & Fakhrurrazi, R. (2018). Antibacterial Activity of Tempe Extract Against Staphylococcus aureus. Jimvet E-ISSN: 2540-9492, 2(3), 230–241. http://jim.unsyiah.ac.id/FKH/article/view/7765
Nafisa, U. (2019). Antioxidant activity test of date palm fruit ethanol extract (Phoenix dactylivera L.). Journal of Farmasindo, 3(2), 1–4.
Nazilah, NRK (2019). Antioxidant Activity Test and Anticancer Potential Screening of Methanol Extract of Ajwa Dates Fruit (Phoenix dactylifera). 65.
Puspitasari, AD, Anwar, FF, & Faizah, NGA (2019). Antioxidant Activity, Determination Of Total Phenolic And Total Flavonoid Levels Of Ethanol, Ethyl Acetate, And n-Hexane Extract Of Petai Leaf (Parkia speciosa Hassk.). Scientific Journal of Teknosains, 5(1), 1.https://doi.org/10.26877/jitek.v5i1.3490
Rizkayanti, Diah, AWM, & Jura, MR (2017). Antioxidant Activity Test of Water Extract and Ethanol Extract of Moringa Leaf (Moringa Oleifera LAM). Journal of Academic Chemistry, 6(2), 125. https://doi.org/10.22487/j24775185.2017.v6.i2.9244
Salim, R. (2018). Antioxidant Activity Test of Purple Leaf Infusion Using DPPH Method (1,1-diphenyl-2- picrylhydrazil). Catalyst Journal, 3(2), 153. https://doi.org/10.22216/jk.v3i2.3372
Sani, IH, Bakar, NHA, Rohin, MAK, Suleiman, I., Umar, MI, & Mohamad, N. (2015). Phoenix dactylifera Linn as a potential novel anti-oxidant in treating major opioid toxicity. Journal of Applied Pharmaceutical Science, 5(8), 167–172. https://doi.org/10.7324/JAPS.2015.50826
Santi, S., & Sicily, TRD (2019). In Vitro Determination Of Antioxidant Activity From Ethanol Propolis Extract Using Dpph Method (1,1-Difenil-2-Picrylhydrazil). Pharmaceutical Media, 7(2), 28–35.
Savitri, I., Suhendra, L., & Wartini, NM (2017). The effect of the type of solvent on the maceration method on the characteristics of the Srgassum polycystum extract. Journal of Agroindustrial Engineering and Management, 5(3), 93–101.
Setyowati, WAE, Ariani, SRD, Ashadi, Mulyani, B., & Rahmawati, CP (2014). Phytochemical Screening and Identification of Main Components of Durian Bark (Durio zibethinus Murr.) Methanol Extract Extract from Petruk Variety. Organic Chemistry of Natural Materials, pp. 271-280. https://www.academia.edu/download/53333198/skrining_literature.pdf
Shahdadi, F., Mirzaei, HO, & Daraei Garmakhany, A. (2015). Study of phenolic compound and antioxidant activity of date fruit as a function of ripening stages and drying process. Journal of Food Science and Technology, 52(3), 1814–1819. https://doi.org/10.1007/s13197-013-1177-6
Ulfah, M., & Sumantri, S. (2014). Test Antioxidant Activity Of Ethanolic Extracts Of Skin Of Lime (Citrus aurantifolia) Fruit With Dpph Method (1,1-diphenyl-2-picrylhydrazil). Faculty of Pharmacy E- Publications, 11(2), 9–17. https://publikasiilmiah.unwahas.ac.id/index.php/Farmasi/article/view/1633
Wufron. (2020). Linear Regression Analysis with IBM SPSS Statistics. March, 0–10. https://doi.org/10.31219/osf.io/fwex8
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